Regulatory

Part:BBa_K112708:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


PfhuA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of <PfhuA> basic part K112708

PCR of dv019/dv020 on   MG1655          (225bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                        (EcoRI/BamHI, 2472+225, L)
Product is pBca1256-K112708
-----------------------------------------------
dv019 Forward biobricking of <PfhuA>      
cgataGAATTCatgAGATCTgctaagcgtgaaataccggatgg

dv020 Reverse biobricking of <PfhuA>
cgttaGGATCCactctgatgtaaagtgaatgataacg

References